Gram-negative bacteria Salmonella typhi are the main cause of typhoid fever not only in developing countries but even in major countries such as the United States of America. Therefore, the World Health Organization (WHO) and health institution have called for infection with this intestinal bacterium to be a warning alaram that may lead to many deaths. The cell wall of bacteria is characterized by distinctive structures, the most important of which is the peptide and glycan layer, due to its importance in the resistance of bacteria to both the immune system and the antibiotics used to eliminate Salmonella bacteria after the Amic enzyme, which is one of hydrolase enzymes, the peptide layer and glycan are essential enzymes that bacteria cannot do without, due to its importance in the division of bacteria and the cellular reconstruction of the peptide and glycan layer. Therefore, the study aimed to investigate the enzymatic activity of the enzyme Amic in the biosynthesis and cleavage of peptidoglycan in Salmonella bacteria, moreover, to test some antibiotics used to treat typhoid and similar diseases on the enzymatic activity of the enzyme Amic by inhibiting or activating that enzyme as anew target for manufacturing or developing novel antibiotics used against Salmonella bacteria.